> For the complete documentation index, see [llms.txt](https://docs.rplpeptides.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://docs.rplpeptides.com/docs/tirzepatide/mass-spectra-library/tirzepatide-mass-spectrometry.md).

# tirzepatide-mass-spectrometry

RPL Peptide

Official Technical Documentation

Mass Spectra Library

***

## Mass Spectrometry Analysis — Tirzepatide

### Analytical Method

| Parameter         | Specification                                   |
| ----------------- | ----------------------------------------------- |
| **Method**        | Liquid Chromatography-Mass Spectrometry (LC-MS) |
| **Ionization**    | Electrospray Ionization (ESI), Positive Mode    |
| **Mass Analyzer** | Quadrupole Time-of-Flight (Q-TOF)               |
| **Scan Range**    | m/z 500–2500                                    |
| **Resolution**    | ≥20,000 (FWHM)                                  |
| **Mass Accuracy** | ±0.5 Da                                         |

### Expected Mass Data

| Parameter                   |                    Value                   |
| --------------------------- | :----------------------------------------: |
| **Molecular Formula**       |               C₂₂₅H₃₄₈N₄₈O₆₈               |
| **Calculated Average Mass** |                 \~4,813 Da                 |
| **Monoisotopic Mass**       |            \[M+H]⁺ = \~4,814 Da            |
| **Charge State Series**     | \[M+3H]³⁺, \[M+4H]⁴⁺, \[M+5H]⁵⁺, \[M+6H]⁶⁺ |

### Acceptance Criteria

| Parameter           |            Limit            |
| ------------------- | :-------------------------: |
| **Mass Accuracy**   | ±0.5 Da of calculated value |
| **Signal-to-Noise** |     ≥10:1 for base peak     |

### Representative Mass Spectrum

*\[Mass spectrum image would be inserted here for each batch]*

### Typical Charge State Distribution

| Charge State | m/z (Calculated) | m/z (Observed) |
| :----------: | :--------------: | :------------: |
|   \[M+3H]³⁺  |         —        |        —       |
|   \[M+4H]⁴⁺  |         —        |        —       |
|   \[M+5H]⁵⁺  |         —        |        —       |
|   \[M+6H]⁶⁺  |         —        |        —       |
|   \[M+7H]⁷⁺  |         —        |        —       |

### Batch-Specific Data

| Batch Number | Observed Mass (Da) | Mass Accuracy (Da) | Status |
| :----------: | :----------------: | :----------------: | :----: |
| \[Batch-001] |          —         |          —         |    —   |

***

### Notes

* Mass spectrometry confirms peptide identity and molecular integrity
* Full mass spectra archived and available upon request
* Deconvolution performed using MaxEnt or similar algorithm

***

© 2026 RPL Peptide

Official Technical Documentation

rplpeptides.com


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