> For the complete documentation index, see [llms.txt](https://docs.rplpeptides.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://docs.rplpeptides.com/docs/tirzepatide/hplc-library/tirzepatide-hplc-analysis.md).

# tirzepatide-hplc-analysis

RPL Peptide

Official Technical Documentation

HPLC Library

***

## HPLC Analysis — Tirzepatide

### Analytical Method

| Parameter                | Specification                                                   |
| ------------------------ | --------------------------------------------------------------- |
| **Method**               | Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) |
| **Detection Wavelength** | 214 nm                                                          |
| **Column**               | C18, 4.6 × 250 mm, 5 μm                                         |
| **Mobile Phase A**       | 0.1% TFA in Water                                               |
| **Mobile Phase B**       | 0.1% TFA in Acetonitrile                                        |
| **Gradient**             | Linear gradient 20–60% B over 30 minutes                        |
| **Flow Rate**            | 1.0 mL/min                                                      |
| **Injection Volume**     | 20 μL                                                           |
| **Column Temperature**   | 25°C                                                            |
| **Run Time**             | 40 minutes                                                      |

### Typical Chromatographic Data

| Parameter                   | Specification |
| --------------------------- | :-----------: |
| **Retention Time**          |   \[Minutes]  |
| **Main Peak Purity**        |     ≥99.0%    |
| **Theoretical Plates**      |    ≥10,000    |
| **Tailing Factor**          |    0.8–1.5    |
| **Relative Retention Time** |     \[RRT]    |

### Acceptance Criteria

| Parameter            |  Limit |
| -------------------- | :----: |
| **Main Peak Purity** | ≥99.0% |
| **Single Impurity**  |  ≤0.5% |
| **Total Impurities** |  ≤1.0% |

### Representative Chromatogram

*\[HPLC chromatogram image would be inserted here for each batch]*

### Batch-Specific Data

| Batch Number | Purity (%) | Retention Time (min) | Impurity Profile |
| :----------: | :--------: | :------------------: | :--------------: |
| \[Batch-001] |      —     |           —          |         —        |

***

### Notes

* HPLC analysis is performed on every batch prior to release
* Chromatograms are archived and available upon request
* All testing follows validated analytical procedures per ICH guidelines

***

© 2026 RPL Peptide

Official Technical Documentation

rplpeptides.com


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