> For the complete documentation index, see [llms.txt](https://docs.rplpeptides.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://docs.rplpeptides.com/docs/semaglutide/01-technical-data-sheet/semaglutide-technical-data-sheet.md). # semaglutide-technical-data-sheet RPL Peptide Official Technical Documentation Technical Data Sheet *** ## Semaglutide — Technical Data Sheet ### 1. General Product Information | Field | Details | | --------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | **Product Name** | Semaglutide | | **CAS Number** | 910463-68-2 | | **Molecular Formula** | C187H291N45O59 | | **Molecular Weight** | 4113.58 Da | | **IUPAC Name** | N-\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-6-amino-1-\[\[(2S)-1-\[(2S)-2-\[\[(2S)-4-carboxy-1-\[\[(2S)-1-\[\[(2S)-5-carbamimidamido-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-5-carbamimidamido-1-\[\[(2S)-1-\[\[(2S)-4-carboxy-1-\[\[(2R)-1-\[\[(2S)-1-\[\[(2S)-5-carbamimidamido-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-5-carbamimidamido-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2R)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-5-carbamimidamido-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-\[\[(2S)-1-hexadecanoylpyrrolidine-2-carbonyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoyl]amino]propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-methylpentanoyl]amino]propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-carboxybutanoyl]amino]propanoyl]amino]-4-methylpentanoyl]amino]-4-carboxybutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]acetyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]acetyl]pyrrolidine-2-carbonyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-methylpentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-methylpentanoyl]amino]propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-methylpentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-methylbutanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-3-hydroxypropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-methylpentanoyl]amino]propanoyl]amino]-4-carboxybutanoyl]amino]-4-carboxybutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-methylpentanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-4-carboxybutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-methylpentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-methylpentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-methylbutanoyl]amino] | > **Note:** The full IUPAC name is provided for chemical reference. For routine laboratory use, refer to the product by its common name Semaglutide or CAS number 910463-68-2. ### 2. Product Specifications | Parameter | Specification | | ----------------------------- | ---------------------------------------------- | | **Peptide Content** | ≥ 80% peptide content (by mass) | | **Purity (HPLC)** | ≥ 99% | | **Appearance** | White to off-white lyophilized powder | | **Salt Form** | Acetate or TFA salt (batch dependent) | | **Solubility** | Soluble in water, PBS, and saline at > 5 mg/mL | | **pH (1% solution in water)** | 4.0 – 7.0 | | **Water Content (KF)** | ≤ 8.0% | | **Endotoxin (LAL)** | ≤ 1.0 EU/mg | | **Molecular Weight (LC-MS)** | 4113.58 ± 1 Da | | **Sequence Confirmation** | Confirmed by MS/MS | ### 3. Sequence Information | Property | Value | | ------------------------- | -------------------------------------------------------------------------------------------------------------------------------- | | **Molecule Type** | Linear peptide | | **Number of Amino Acids** | 31 | | **Amino Acid Sequence** | H-His-Aib-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys-Glu-Phe-Ile-Ala-Trp-Leu-Val-Arg-Gly-Arg-Gly-OH | | **Modifications** | C-18 fatty diacid (hexadecanedioic acid) conjugated to Lys at position 20 via a hydrophilic linker (gamma-Glu-2xOEG) | | **Disulfide Bonds** | None | | **Secondary Structure** | Partial alpha-helix in solution | ### 4. Activity Profile | Property | Value | | --------------------------- | ------------------------------------------------ | | **Target** | GLP-1 Receptor | | **Receptor Type** | Class B G protein-coupled receptor (GPCR) | | **Mechanism** | Full agonist | | **Half-life** | Approximately 165 hours (7 days) in human plasma | | **Binding Affinity (IC50)** | \~ 0.5 nM for human GLP-1 receptor | | **cAMP Potency (EC50)** | \~ 0.5 – 1.5 nM | ### 5. Quality Assurance RPL Peptide ensures the following quality control measures for all Semaglutide batches: * **Purity Analysis:** Reverse-phase HPLC with UV detection at 214 nm and 280 nm * **Mass Confirmation:** LC-MS for intact molecular weight determination * **Sequence Confirmation:** MS/MS fragmentation analysis * **Water Content:** Karl Fischer titration * **Endotoxin Testing:** LAL gel-clot method * **Appearance:** Visual inspection * **Residual TFA:** Ion chromatography (where applicable) * **Peptide Content:** Amino acid analysis or UV quantitation Each batch is accompanied by a Certificate of Analysis (COA) detailing all measured parameters. ### 6. Reconstitution Protocol | Step | Action | | ---- | ------------------------------------------------------------------------------------- | | 1 | Equilibrate the vial to room temperature for 15 minutes | | 2 | Gently tap the vial to ensure all powder settles at the bottom | | 3 | Remove flip-off cap and disinfect the rubber septum with 70% isopropanol | | 4 | Slowly inject sterile water for injection, PBS, or sterile saline along the vial wall | | 5 | Swirl gently — **do not vortex** — until completely dissolved | | 6 | If needed, further dilute in appropriate buffer for the specific assay | #### Reconstitution Volume Table | Bottle Size | Recommended Reconstitution Volume | | ----------- | --------------------------------- | | 10 mg | 1.0 – 2.0 mL | | 15 mg | 1.5 – 3.0 mL | | 20 mg | 2.0 – 4.0 mL | | 30 mg | 3.0 – 6.0 mL | | 40 mg | 4.0 – 8.0 mL | | 50 mg | 5.0 – 10.0 mL | | 60 mg | 6.0 – 12.0 mL | | 100 mg | 10.0 – 20.0 mL | | 120 mg | 12.0 – 24.0 mL | ### 7. Storage Conditions | Condition | Specification | | ---------------------------- | ---------------------------------------------- | | **Lyophilized (Long-term)** | -20 °C, desiccated, protected from light | | **Lyophilized (Short-term)** | 2 – 8 °C for up to 4 weeks | | **Reconstituted** | 2 – 8 °C for up to 7 days | | **Aliquoted and Frozen** | -20 °C for up to 4 weeks (single-use aliquots) | | **Transport** | Cold chain (dry ice or gel packs) | ### 8. Regulatory & Safety Classification | Classification | Status | | ---------------------------- | ------------------------------------- | | **For Human Use** | No — laboratory research use only | | **For Veterinary Use** | No | | **Hazard Classification** | Not classified as hazardous under GHS | | **UN Number** | Not applicable | | **Transport Classification** | Not regulated as dangerous goods | ### 9. Disclaimer **FOR LABORATORY RESEARCH USE ONLY. NOT FOR HUMAN OR VETERINARY USE.** This technical data sheet is provided for informational purposes only. RPL Peptide makes no warranties, express or implied, regarding the accuracy, completeness, or suitability of the information provided. The user assumes all risk and liability associated with the use of this product. It is the responsibility of the user to ensure that the product is used in compliance with all applicable laws, regulations, and institutional guidelines. *** © 2026 RPL Peptide Official Technical Documentation rplpeptides.com --- # Agent Instructions This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com. ## Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. 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